File:Rgnef-(p190RhoGEF)-Knockout-Inhibits-RhoA-Activity-Focal-Adhesion-Establishment-and-Cell-Motility-pone.0037830.s003.ogv
Rgnef-(p190RhoGEF)-Knockout-Inhibits-RhoA-Activity-Focal-Adhesion-Establishment-and-Cell-Motility-pone.0037830.s003.ogv (Ogg Theora video file, length 3.3 s, 672 × 512 pixels, 6.58 Mbps, file size: 2.59 MB)
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[edit]DescriptionRgnef-(p190RhoGEF)-Knockout-Inhibits-RhoA-Activity-Focal-Adhesion-Establishment-and-Cell-Motility-pone.0037830.s003.ogv |
English: Rgnef+/+ MEF scratch wound motility. Primary normal (Rgnef+/+) MEFs were plated at a subconfluent density (75%) on glass bottom dishes (MatTek) coated with FN (1 µg/ml). After 24 h, cells were serum starved overnight, wounded with a pipette tip, washed with PBS, and incubated in growth media containing 0.5 µg/ml of mitomycin-C prior to phase contrast imaging every 15 min in a humidified 5% CO2 environment at 37°C using an Olympus IX51 microscope, XY-controlled stage with Z focus drive (Olympus), 10× objective (UPLFL, 0.30 NA), and an OrcaER camera (Hamamatsu) controlled by Slidebook (v5.0) software. Images were acquired every 15 minutes and assembled into 10 frames per second video spanning 8 h. |
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Source | Video S1 from Miller N, Lawson C, Chen X, Lim S, Schlaepfer D (2012). "Rgnef (p190RhoGEF) Knockout Inhibits RhoA Activity, Focal Adhesion Establishment, and Cell Motility Downstream of Integrins". PLOS ONE. DOI:10.1371/journal.pone.0037830. PMID 22649559. PMC: 3359313. | ||
Author | Miller N, Lawson C, Chen X, Lim S, Schlaepfer D | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:21, 30 October 2012 | 3.3 s, 672 × 512 (2.59 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Miller N, Lawson C, Chen X, Lim S, Schlaepfer D |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |