UDP-N-acetilglukozaminska 2-epimeraza (hidroliza)
UDP-N-acetilglukozaminska 2-epimeraza (hidroliza) (EC 3.2.1.183, GNE (gen), siaA (gen), neuC (gen)) je enzim sa sistematskim imenom UDP-N-acetil-alfa-D-glukozamin hidrolaza (2-epimerizacija).[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
UDP-N-acetilglukozaminska 2-epimeraza (hidroliza) | |||||||||
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Identifikatori | |||||||||
EC broj | 3.2.1.183 | ||||||||
Baze podataka | |||||||||
IntEnz | IntEnz pregled | ||||||||
BRENDA | BRENDA pristup | ||||||||
ExPASy | NiceZyme pregled | ||||||||
KEGG | KEGG pristup | ||||||||
MetaCyc | metabolički put | ||||||||
PRIAM | profil | ||||||||
Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- UDP-N-acetil-alfa-D-glukozamin + H2O N-acetil-D-manozamin + UDP
Enzim je izdvojen iz jetre sisara, kao i iz nekih patogenih bakterija uključujući Neisseria meningitidis i Staphylococcus aureus.
Reference
уреди- ^ Stasche, R., Hinderlich, S., Weise, C., Effertz, K., Lucka, L., Moormann, P. and Reutter, W. (1997). „A bifunctional enzyme catalyzes the first two steps in N-acetylneuraminic acid biosynthesis of rat liver. Molecular cloning and functional expression of UDP-N-acetyl-glucosamine 2-epimerase/N-acetylmannosamine kinase”. J. Biol. Chem. 272: 24319—24324. PMID 9305888.
- ^ Chou, W.K., Hinderlich, S., Reutter, W. and Tanner, M.E. (2003). „Sialic acid biosynthesis: stereochemistry and mechanism of the reaction catalyzed by the mammalian UDP-N-acetylglucosamine 2-epimerase”. J. Am. Chem. Soc. 125: 2455—2461. PMID 12603133.
- ^ Blume, A., Ghaderi, D., Liebich, V., Hinderlich, S., Donner, P., Reutter, W. and Lucka, L. (2004). „UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, functionally expressed in and purified from Escherichia coli, yeast, and insect cells”. Protein Expr. Purif. 35: 387—396. PMID 15135418.
- ^ Murkin, A.S., Chou, W.K., Wakarchuk, W.W. and Tanner, M.E. (2004). „Identification and mechanism of a bacterial hydrolyzing UDP-N-acetylglucosamine 2-epimerase”. Biochemistry. 43: 14290—14298. PMID 15518580.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze
уреди- UDP-N-acetylglucosamine+2-epimerase+(hydrolysing) на US National Library of Medicine Medical Subject Headings (MeSH)