A group of regional residents called Protect the Kobuk has organized opposition to the road in the [[Northwest Arctic Borough]].<ref name=":3">{{Cite web |title=Protect the Kobuk |url=https://www.protectthekobuk.org/ |url-status=live |archive-url=https://web.archive.org/web/20240516104723/https://www.protectthekobuk.org/ |archive-date=May 16, 2024}}</ref>
In addition to Protect the Kobuk, the several notable organizations have publicly opposed the project, including the [[Tanana Chiefs Conference]]<ref>{{Cite web |title=Stop the Ambler Road |url=https://www.tananachiefs.org/get-involved/ambler-road-project/ |url-status=live |archive-url=https://web.archive.org/web/20231024012304/https://www.tananachiefs.org/get-involved/ambler-road-project/ |archive-date=October 24, 2023 |access-date=May 3, 2024 |website=[[Tanana Chiefs Conference]]}}</ref>, the [[Sierra Club]]<ref>{{Cite web |date=April 18, 2024 |title=Biden Administration Moves to Block Proposed Ambler Road |url=https://www.sierraclub.org/press-releases/2024/04/biden-administration-moves-block-proposed-ambler-road |url-status=live |archive-url=https://web.archive.org/web/20240627232654/https://www.sierraclub.org/press-releases/2024/04/biden-administration-moves-block-proposed-ambler-road |archive-date=June 27, 2024 |website=[[Sierra Club]]}}</ref>, the [[Center for American Progress|Center for American Progess]]<ref>{{Cite web |date=April 19, 2024 |title=STATEMENT: Biden Administration Protects Vulnerable Lands in America’s Arctic |url=https://www.americanprogress.org/press/statement-biden-administration-protects-vulnerable-lands-in-americas-arctic/ |url-status=live |archive-url=https://web.archive.org/web/20240627232204/https://www.americanprogress.org/press/statement-biden-administration-protects-vulnerable-lands-in-americas-arctic/ |archive-date=June 27, 2024 |website=[[Center for American Progress]]}}</ref>, and numerous other environmental and [[Alaska Natives|Alaska Native]] organizations.<ref name=":2" />